rabbit anti a3g  (Novus Biologicals)

Journal: Frontiers in immunology

Article Title: APOBEC3G/3A Expression in Human Immunodeficiency Virus Type 1-Infected Individuals Following Initiation of Antiretroviral Therapy Containing Cenicriviroc or Efavirenz.

doi: 10.3389/fimmu.2018.01839

Figure Lengend Snippet: FIGURE 1 | A3G expression in cenicriviroc (CVC) and efavirenz (EFV) arms and correlation with surrogate markers of disease progression. (A) Expression of A3G in peripheral blood mononuclear cells of subjects treated with CVC (circles) or EFV (triangles). The dot plots show the ratios of A3G to actin OD determined by densitometry for each time point of the patients analyzed (CVC: n = 21 baseline, n = 19 week 4, n = 18 week 12, n = 20 week 24, n = 23 week 48; EFV: n = 9 baseline, n = 10 week 4, n = 9 week 12, n = 10 week 24, n = 9 week 48). Median values (50th percentiles) are shown by the horizontal bars. (B) Correlation between baseline A3G levels and CD4+ cell counts (n = 30). (C) Correlation between baseline A3G levels and week 48 CD4+/CD8+ cell ratio (n = 29; CVC: n = 21; EFV: n = 8). Gray circles and black triangles, CVC- and EFV-treated subjects, respectively. Statistical analysis was done using the Wilcoxon signed-rank test (A) and the non-parametric one-tailed Spearman’s test to determine correlation coefficients (B,C).

Article Snippet: Membranes were incubated with 4–5% fat-free milk dissolved in PBS-T (PBS 1×, 0.05%) to block non-specific binding and then probed with the following Abs: anti-human A3A (rabbit polyclonal D23, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-human A3G (rabbit polyclonal D9C6Z, Cell Signaling Technology, Beverly, MA, USA), and anti-actin (mouse monoclonal Abs-5, BD Biosciences, San Diego, CA, USA) as gel loading control.

Techniques: Expressing, Biomarker Discovery, One-tailed Test